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bio rad cfx 193 opus 96  (Bio-Rad)


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    Bio-Rad bio rad cfx 193 opus 96
    Bio Rad Cfx 193 Opus 96, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 22524 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bio+rad+cfx+opus+96/CFX96+Touch+Deep+Well+Real-Time+PCR+Detection+System/pm42096515-90-12-12
    Average 96 stars, based on 22524 article reviews
    bio rad cfx 193 opus 96 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: Identification of Hunnivirus in Bovine and Caprine Samples in North America
    Article Snippet: .. Real-time RT-PCR was run on the Bio-Rad CFX Opus 96 Real-Time thermocycler (Bio-Rad, Hercules, CA, USA) with the following conditions: UNG incubation at 25 °C for 2 min, reverse transcription at 48 °C for 10 min, PCR activation and reaction at 95 °C for 10 min, and 40 cycles of 95 °C for 15 s and 60 °C for 45 s Results were analyzed using the CFX Maestro software (Bio-Rad, Hercules, CA, USA, version 1.0). .. Conventional RT-PCRs were performed using the SuperScriptTM III One-Step RT-PCR System with PlatinumTM Taq DNA Polymerase (Invitrogen, Carlsbad, CA, USA, cat. no. 12574026).

    Article Title: Identification of Hunnivirus in Bovine and Caprine Samples in North America
    Article Snippet: .. Real-time RT-PCR was run on the Bio-Rad CFX Opus 96 Real-Time thermocycler (Bio-Rad, Hercules, CA, USA) with the following conditions: UNG incubation at 25 ◦C for 2 min, reverse transcription at 48 ◦C for 10 min, PCR activation and reaction at 95 ◦C for 10 min, and 40 cycles of 95 ◦C for 15 s and 60 ◦C for 45 s Results were analyzed using the CFX Maestro software (Bio-Rad, Hercules, CA, USA, version 1.0). .. Conventional RT-PCRs were performed using the SuperScriptTM III One-Step RT-PCR System with PlatinumTM Taq DNA Polymerase (Invitrogen, Carlsbad, CA, USA, cat. no. 12574026).

    Incubation:

    Article Title: Identification of Hunnivirus in Bovine and Caprine Samples in North America
    Article Snippet: .. Real-time RT-PCR was run on the Bio-Rad CFX Opus 96 Real-Time thermocycler (Bio-Rad, Hercules, CA, USA) with the following conditions: UNG incubation at 25 °C for 2 min, reverse transcription at 48 °C for 10 min, PCR activation and reaction at 95 °C for 10 min, and 40 cycles of 95 °C for 15 s and 60 °C for 45 s Results were analyzed using the CFX Maestro software (Bio-Rad, Hercules, CA, USA, version 1.0). .. Conventional RT-PCRs were performed using the SuperScriptTM III One-Step RT-PCR System with PlatinumTM Taq DNA Polymerase (Invitrogen, Carlsbad, CA, USA, cat. no. 12574026).

    Article Title: Identification of Hunnivirus in Bovine and Caprine Samples in North America
    Article Snippet: .. Real-time RT-PCR was run on the Bio-Rad CFX Opus 96 Real-Time thermocycler (Bio-Rad, Hercules, CA, USA) with the following conditions: UNG incubation at 25 ◦C for 2 min, reverse transcription at 48 ◦C for 10 min, PCR activation and reaction at 95 ◦C for 10 min, and 40 cycles of 95 ◦C for 15 s and 60 ◦C for 45 s Results were analyzed using the CFX Maestro software (Bio-Rad, Hercules, CA, USA, version 1.0). .. Conventional RT-PCRs were performed using the SuperScriptTM III One-Step RT-PCR System with PlatinumTM Taq DNA Polymerase (Invitrogen, Carlsbad, CA, USA, cat. no. 12574026).

    Reverse Transcription:

    Article Title: Identification of Hunnivirus in Bovine and Caprine Samples in North America
    Article Snippet: .. Real-time RT-PCR was run on the Bio-Rad CFX Opus 96 Real-Time thermocycler (Bio-Rad, Hercules, CA, USA) with the following conditions: UNG incubation at 25 °C for 2 min, reverse transcription at 48 °C for 10 min, PCR activation and reaction at 95 °C for 10 min, and 40 cycles of 95 °C for 15 s and 60 °C for 45 s Results were analyzed using the CFX Maestro software (Bio-Rad, Hercules, CA, USA, version 1.0). .. Conventional RT-PCRs were performed using the SuperScriptTM III One-Step RT-PCR System with PlatinumTM Taq DNA Polymerase (Invitrogen, Carlsbad, CA, USA, cat. no. 12574026).

    Article Title: Identification of Hunnivirus in Bovine and Caprine Samples in North America
    Article Snippet: .. Real-time RT-PCR was run on the Bio-Rad CFX Opus 96 Real-Time thermocycler (Bio-Rad, Hercules, CA, USA) with the following conditions: UNG incubation at 25 ◦C for 2 min, reverse transcription at 48 ◦C for 10 min, PCR activation and reaction at 95 ◦C for 10 min, and 40 cycles of 95 ◦C for 15 s and 60 ◦C for 45 s Results were analyzed using the CFX Maestro software (Bio-Rad, Hercules, CA, USA, version 1.0). .. Conventional RT-PCRs were performed using the SuperScriptTM III One-Step RT-PCR System with PlatinumTM Taq DNA Polymerase (Invitrogen, Carlsbad, CA, USA, cat. no. 12574026).

    Polymerase Chain Reaction:

    Article Title: Identification of Hunnivirus in Bovine and Caprine Samples in North America
    Article Snippet: .. Real-time RT-PCR was run on the Bio-Rad CFX Opus 96 Real-Time thermocycler (Bio-Rad, Hercules, CA, USA) with the following conditions: UNG incubation at 25 °C for 2 min, reverse transcription at 48 °C for 10 min, PCR activation and reaction at 95 °C for 10 min, and 40 cycles of 95 °C for 15 s and 60 °C for 45 s Results were analyzed using the CFX Maestro software (Bio-Rad, Hercules, CA, USA, version 1.0). .. Conventional RT-PCRs were performed using the SuperScriptTM III One-Step RT-PCR System with PlatinumTM Taq DNA Polymerase (Invitrogen, Carlsbad, CA, USA, cat. no. 12574026).

    Article Title: Identification of Hunnivirus in Bovine and Caprine Samples in North America
    Article Snippet: .. Real-time RT-PCR was run on the Bio-Rad CFX Opus 96 Real-Time thermocycler (Bio-Rad, Hercules, CA, USA) with the following conditions: UNG incubation at 25 ◦C for 2 min, reverse transcription at 48 ◦C for 10 min, PCR activation and reaction at 95 ◦C for 10 min, and 40 cycles of 95 ◦C for 15 s and 60 ◦C for 45 s Results were analyzed using the CFX Maestro software (Bio-Rad, Hercules, CA, USA, version 1.0). .. Conventional RT-PCRs were performed using the SuperScriptTM III One-Step RT-PCR System with PlatinumTM Taq DNA Polymerase (Invitrogen, Carlsbad, CA, USA, cat. no. 12574026).

    Activation Assay:

    Article Title: Identification of Hunnivirus in Bovine and Caprine Samples in North America
    Article Snippet: .. Real-time RT-PCR was run on the Bio-Rad CFX Opus 96 Real-Time thermocycler (Bio-Rad, Hercules, CA, USA) with the following conditions: UNG incubation at 25 °C for 2 min, reverse transcription at 48 °C for 10 min, PCR activation and reaction at 95 °C for 10 min, and 40 cycles of 95 °C for 15 s and 60 °C for 45 s Results were analyzed using the CFX Maestro software (Bio-Rad, Hercules, CA, USA, version 1.0). .. Conventional RT-PCRs were performed using the SuperScriptTM III One-Step RT-PCR System with PlatinumTM Taq DNA Polymerase (Invitrogen, Carlsbad, CA, USA, cat. no. 12574026).

    Article Title: Identification of Hunnivirus in Bovine and Caprine Samples in North America
    Article Snippet: .. Real-time RT-PCR was run on the Bio-Rad CFX Opus 96 Real-Time thermocycler (Bio-Rad, Hercules, CA, USA) with the following conditions: UNG incubation at 25 ◦C for 2 min, reverse transcription at 48 ◦C for 10 min, PCR activation and reaction at 95 ◦C for 10 min, and 40 cycles of 95 ◦C for 15 s and 60 ◦C for 45 s Results were analyzed using the CFX Maestro software (Bio-Rad, Hercules, CA, USA, version 1.0). .. Conventional RT-PCRs were performed using the SuperScriptTM III One-Step RT-PCR System with PlatinumTM Taq DNA Polymerase (Invitrogen, Carlsbad, CA, USA, cat. no. 12574026).

    Maestro Software:

    Article Title: Identification of Hunnivirus in Bovine and Caprine Samples in North America
    Article Snippet: .. Real-time RT-PCR was run on the Bio-Rad CFX Opus 96 Real-Time thermocycler (Bio-Rad, Hercules, CA, USA) with the following conditions: UNG incubation at 25 °C for 2 min, reverse transcription at 48 °C for 10 min, PCR activation and reaction at 95 °C for 10 min, and 40 cycles of 95 °C for 15 s and 60 °C for 45 s Results were analyzed using the CFX Maestro software (Bio-Rad, Hercules, CA, USA, version 1.0). .. Conventional RT-PCRs were performed using the SuperScriptTM III One-Step RT-PCR System with PlatinumTM Taq DNA Polymerase (Invitrogen, Carlsbad, CA, USA, cat. no. 12574026).

    Article Title: Evaluation of RT-LAMP for SARS-CoV-2 Detection in Animal Feces
    Article Snippet: rRT-PCR was performed using the AgPath-IDTM One-Step RT-PCR kit (Thermo Fisher Scientific, Waltham, MA, USA), utilizing 12.5 μL of 2X reaction buffer, 1.0 μL of XenoTM, 1.0 μL of LIZ assay, 1.0 μL of 25X RT-PCR enzyme mix, 5.5 μL of biological-grade water, and 2.0 μL of CDC N1 primer/probe mix for each sample for the rRT-PCR Master Mix, which totaled 23.0 μL [ ]. .. For each sample, 2.0 μL of extracted RNA was added to match the amount of template utilized in the RT-LAMP assay, for a grand total of 25.0 μL. rRT-PCR was performed for 40 amplification cycles on a Bio-Rad CFX Opus 96 Real-Time PCR instrument using Bio-Rad CFX Maestro software 2.3 (Bio-Rad Laboratories, Hercules, CA, USA). ..

    Article Title: Evaluation of Fecal Sample Pooling for Real-Time RT-PCR Testing SARS-CoV-2 in Animals
    Article Snippet: The nucleic acids of individual and pooled samples were extracted using the MagMAXTM Pathogen RNA/DNA Kit on a KingFisher Flex machine (ThermoFisher, Waltham, MA, USA). .. An amount of 200 μL of the sample for extraction and 60 μL of an elution buffer was used. rRT-PCR was performed on a Bio-Rad CFX Opus 96 Real-Time PCR instrument (BioRad Laboratories, Inc., Hercules, CA, USA) and Bio-Rad CFX Maestro software 2.3 using the CDC-based N1 primers and probe for SARS-CoV-2 [24]. ..

    Article Title: Identification of Hunnivirus in Bovine and Caprine Samples in North America
    Article Snippet: .. Real-time RT-PCR was run on the Bio-Rad CFX Opus 96 Real-Time thermocycler (Bio-Rad, Hercules, CA, USA) with the following conditions: UNG incubation at 25 ◦C for 2 min, reverse transcription at 48 ◦C for 10 min, PCR activation and reaction at 95 ◦C for 10 min, and 40 cycles of 95 ◦C for 15 s and 60 ◦C for 45 s Results were analyzed using the CFX Maestro software (Bio-Rad, Hercules, CA, USA, version 1.0). .. Conventional RT-PCRs were performed using the SuperScriptTM III One-Step RT-PCR System with PlatinumTM Taq DNA Polymerase (Invitrogen, Carlsbad, CA, USA, cat. no. 12574026).

    Article Title: Evaluation of RT-LAMP for SARS-CoV-2 Detection in Animal Feces.
    Article Snippet: rRT-PCR was performed using the AgPath-IDTM One-Step RT-PCR kit (Thermo Fisher Scientific, Waltham, MA, USA), utilizing 12.5 μL of 2X reaction buffer, 1.0 μL of XenoTM, 1.0 μL of LIZ assay, 1.0 μL of 25X RT-PCR enzyme mix, 5.5 μL of biological-grade water, and 2.0 μL of CDC N1 primer/probe mix for each sample for the rRT-PCR Master Mix, which totaled 23.0 μL [30]. .. For each sample, 2.0 μL of extracted RNA was added to match the amount of template utilized in the RT-LAMP assay, for a grand total of 25.0 μL. rRT-PCR was performed for 40 amplification cycles on a Bio-Rad CFX Opus 96 Real-Time PCR instrument using Bio-Rad CFX Maestro software 2.3 (Bio-Rad Laboratories, Hercules, CA, USA). ..

    RT Lamp Assay:

    Article Title: Evaluation of RT-LAMP for SARS-CoV-2 Detection in Animal Feces
    Article Snippet: rRT-PCR was performed using the AgPath-IDTM One-Step RT-PCR kit (Thermo Fisher Scientific, Waltham, MA, USA), utilizing 12.5 μL of 2X reaction buffer, 1.0 μL of XenoTM, 1.0 μL of LIZ assay, 1.0 μL of 25X RT-PCR enzyme mix, 5.5 μL of biological-grade water, and 2.0 μL of CDC N1 primer/probe mix for each sample for the rRT-PCR Master Mix, which totaled 23.0 μL [ ]. .. For each sample, 2.0 μL of extracted RNA was added to match the amount of template utilized in the RT-LAMP assay, for a grand total of 25.0 μL. rRT-PCR was performed for 40 amplification cycles on a Bio-Rad CFX Opus 96 Real-Time PCR instrument using Bio-Rad CFX Maestro software 2.3 (Bio-Rad Laboratories, Hercules, CA, USA). ..

    Article Title: Evaluation of RT-LAMP for SARS-CoV-2 Detection in Animal Feces.
    Article Snippet: rRT-PCR was performed using the AgPath-IDTM One-Step RT-PCR kit (Thermo Fisher Scientific, Waltham, MA, USA), utilizing 12.5 μL of 2X reaction buffer, 1.0 μL of XenoTM, 1.0 μL of LIZ assay, 1.0 μL of 25X RT-PCR enzyme mix, 5.5 μL of biological-grade water, and 2.0 μL of CDC N1 primer/probe mix for each sample for the rRT-PCR Master Mix, which totaled 23.0 μL [30]. .. For each sample, 2.0 μL of extracted RNA was added to match the amount of template utilized in the RT-LAMP assay, for a grand total of 25.0 μL. rRT-PCR was performed for 40 amplification cycles on a Bio-Rad CFX Opus 96 Real-Time PCR instrument using Bio-Rad CFX Maestro software 2.3 (Bio-Rad Laboratories, Hercules, CA, USA). ..

    Amplification:

    Article Title: Evaluation of RT-LAMP for SARS-CoV-2 Detection in Animal Feces
    Article Snippet: rRT-PCR was performed using the AgPath-IDTM One-Step RT-PCR kit (Thermo Fisher Scientific, Waltham, MA, USA), utilizing 12.5 μL of 2X reaction buffer, 1.0 μL of XenoTM, 1.0 μL of LIZ assay, 1.0 μL of 25X RT-PCR enzyme mix, 5.5 μL of biological-grade water, and 2.0 μL of CDC N1 primer/probe mix for each sample for the rRT-PCR Master Mix, which totaled 23.0 μL [ ]. .. For each sample, 2.0 μL of extracted RNA was added to match the amount of template utilized in the RT-LAMP assay, for a grand total of 25.0 μL. rRT-PCR was performed for 40 amplification cycles on a Bio-Rad CFX Opus 96 Real-Time PCR instrument using Bio-Rad CFX Maestro software 2.3 (Bio-Rad Laboratories, Hercules, CA, USA). ..

    Article Title: Evaluation of RT-LAMP for SARS-CoV-2 Detection in Animal Feces.
    Article Snippet: rRT-PCR was performed using the AgPath-IDTM One-Step RT-PCR kit (Thermo Fisher Scientific, Waltham, MA, USA), utilizing 12.5 μL of 2X reaction buffer, 1.0 μL of XenoTM, 1.0 μL of LIZ assay, 1.0 μL of 25X RT-PCR enzyme mix, 5.5 μL of biological-grade water, and 2.0 μL of CDC N1 primer/probe mix for each sample for the rRT-PCR Master Mix, which totaled 23.0 μL [30]. .. For each sample, 2.0 μL of extracted RNA was added to match the amount of template utilized in the RT-LAMP assay, for a grand total of 25.0 μL. rRT-PCR was performed for 40 amplification cycles on a Bio-Rad CFX Opus 96 Real-Time PCR instrument using Bio-Rad CFX Maestro software 2.3 (Bio-Rad Laboratories, Hercules, CA, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Evaluation of RT-LAMP for SARS-CoV-2 Detection in Animal Feces
    Article Snippet: rRT-PCR was performed using the AgPath-IDTM One-Step RT-PCR kit (Thermo Fisher Scientific, Waltham, MA, USA), utilizing 12.5 μL of 2X reaction buffer, 1.0 μL of XenoTM, 1.0 μL of LIZ assay, 1.0 μL of 25X RT-PCR enzyme mix, 5.5 μL of biological-grade water, and 2.0 μL of CDC N1 primer/probe mix for each sample for the rRT-PCR Master Mix, which totaled 23.0 μL [ ]. .. For each sample, 2.0 μL of extracted RNA was added to match the amount of template utilized in the RT-LAMP assay, for a grand total of 25.0 μL. rRT-PCR was performed for 40 amplification cycles on a Bio-Rad CFX Opus 96 Real-Time PCR instrument using Bio-Rad CFX Maestro software 2.3 (Bio-Rad Laboratories, Hercules, CA, USA). ..

    Article Title: Evaluation of Fecal Sample Pooling for Real-Time RT-PCR Testing SARS-CoV-2 in Animals
    Article Snippet: The nucleic acids of individual and pooled samples were extracted using the MagMAXTM Pathogen RNA/DNA Kit on a KingFisher Flex machine (ThermoFisher, Waltham, MA, USA). .. An amount of 200 μL of the sample for extraction and 60 μL of an elution buffer was used. rRT-PCR was performed on a Bio-Rad CFX Opus 96 Real-Time PCR instrument (BioRad Laboratories, Inc., Hercules, CA, USA) and Bio-Rad CFX Maestro software 2.3 using the CDC-based N1 primers and probe for SARS-CoV-2 [24]. ..

    Article Title: Evaluation of RT-LAMP for SARS-CoV-2 Detection in Animal Feces.
    Article Snippet: rRT-PCR was performed using the AgPath-IDTM One-Step RT-PCR kit (Thermo Fisher Scientific, Waltham, MA, USA), utilizing 12.5 μL of 2X reaction buffer, 1.0 μL of XenoTM, 1.0 μL of LIZ assay, 1.0 μL of 25X RT-PCR enzyme mix, 5.5 μL of biological-grade water, and 2.0 μL of CDC N1 primer/probe mix for each sample for the rRT-PCR Master Mix, which totaled 23.0 μL [30]. .. For each sample, 2.0 μL of extracted RNA was added to match the amount of template utilized in the RT-LAMP assay, for a grand total of 25.0 μL. rRT-PCR was performed for 40 amplification cycles on a Bio-Rad CFX Opus 96 Real-Time PCR instrument using Bio-Rad CFX Maestro software 2.3 (Bio-Rad Laboratories, Hercules, CA, USA). ..

    Extraction:

    Article Title: Evaluation of Fecal Sample Pooling for Real-Time RT-PCR Testing SARS-CoV-2 in Animals
    Article Snippet: The nucleic acids of individual and pooled samples were extracted using the MagMAXTM Pathogen RNA/DNA Kit on a KingFisher Flex machine (ThermoFisher, Waltham, MA, USA). .. An amount of 200 μL of the sample for extraction and 60 μL of an elution buffer was used. rRT-PCR was performed on a Bio-Rad CFX Opus 96 Real-Time PCR instrument (BioRad Laboratories, Inc., Hercules, CA, USA) and Bio-Rad CFX Maestro software 2.3 using the CDC-based N1 primers and probe for SARS-CoV-2 [24]. ..



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